褪黑素引发对旱地小麦种子萌发期抗旱性的影响OA
Effects of melatonin priming on drought resistance of dryland wheat at seed germination stage
为明确褪黑素对旱地小麦(Triticum aestivum L.)种子萌发期抗旱性的调节作用,以旱地小麦品种商麦5226为试验材料,测定不同浓度(0、75、150、300 μmol/L)褪黑素引发处理后在不同干旱强度[聚乙二醇(PEG-6000)浓度分别为0、10%、15%、20%和25%]下种子萌发指标和幼苗生理指标的变化.结果表明,随着干旱胁迫的加剧,发芽势、发芽率、幼苗含水量、胚芽长、胚根长、胚根数和叶绿素含量均呈下降趋势,可溶性糖和脯氨酸含量整体上均呈增加趋势.与对应干旱胁迫处理下的无引发处理相比,75、150 μmol/L褪黑素引发后10%、15%和20%PEG-6000下发芽势和发芽率均有不同程度的增加(除20%PEG-6000下发芽率外),75、150、300 μmol/L褪黑素引发显著增加了 10%和15%PEG-6000处理下可溶性糖含量.隶属函数法综合评价表明,75、150μmol/L褪黑素引发下10%和15%PEG-6000处理、300 μmol/L褪黑素引发下15%和25%PEG-6000处理的综合评价值高于其对应干旱胁迫处理下的无引发处理.综合来看,75、150 μmol/L褪黑素引发可有效缓解10%和15%PEG-6000强度的干旱胁迫.
In order to clarify the regulatory effect of melatonin on the drought resistance of dryland wheat(Triticum aestivum L.)dur-ing seed germination stage,the dryland wheat variety Shangmai 5226 was used as the experimental material to determine the effects of different concentrations(0,75,150 and 300 μmol/L)of melatonin priming on seed germination and seedling physiological indexes under different drought intensities[0,10%,15%,20%and 25%of polyethylene glycol(PEG-6000)].The results indicated that with the intensification of drought stress,germination potential,germination rate,seedling water content,coleoptile length,radicle length,radicle number,and chlorophyll content showed a decreasing trend,while soluble sugar and proline contents increased.Com-pared with the drought-stressed treatments that did not receive priming,melatonin priming at 75 and 150 μmol/L increased germina-tion potential and germination rate to varying degrees under 10%,15%,and 20%PEG-6000 treatments(except for germination rate under 20%PEG-6000),and melatonin priming at 75,150,and 300 μmol/L significantly enhanced soluble sugar content under 10%and 15%PEG-6000 treatments.The comprehensive evaluation using the membership function method showed that under melatonin priming at 75 and 150 μmol/L,the comprehensive evaluation values for the 10%and 15%PEG-6000 treatments were higher than those of the corresponding drought-stressed treatments without priming;similarly,under 300 μmol/L melatonin priming,the values for the 15%and 25%PEG-6000 treatments were higher than those of their respective controls without priming.In conclusion,75 and 150 μmol/L melatonin priming could effectively alleviate the drought stress of 10%and 15%PEG-6000 intensities.
吴秀宁;汤云霞;段小平;高靖萱;高宝云;张军;王新军
商洛学院,健康管理学院/陕西省"四主体一联合"秦岭康养产业校企联合研究中心,陕西商洛 726000商南县农业技术服务中心,陕西商南 726300商南县农业技术服务中心,陕西商南 726300商洛学院,生物医药与食品工程学院,陕西商洛 726000商洛学院,生物医药与食品工程学院,陕西商洛 726000商洛学院,生物医药与食品工程学院,陕西商洛 726000商洛学院,生物医药与食品工程学院,陕西商洛 726000
农业科技
旱地小麦(Triticum aestivum L.)褪黑素种子引发萌发期抗旱性
dryland wheat(Triticum aestivum L.)melatoninseed primingseed germination stagedrought resistance
《湖北农业科学》 2026 (2)
1-4,8,5
陕西省科技厅项目(2022FP-33)商洛市科技计划项目(2022-Z-0027)商洛学院博士科研发展基金项目(25SKY006)
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