无外源交联剂的丝素蛋白/透明质酸可注射水凝胶的构建与表征OA

In view of the problem that the current hydrogel materials need to rely on chemical crosslinking agents to make up for their insufficient mechanical properties in the biomedical field,silk fibroin(SF)and hyaluronic acid were modified to construct a material system that can form hydrogels without external crosslinking agents.Firstly,polyethyleneimine(PEI)was used for amino(—NH2)grafting modification of SF,and sodium periodate(NaIO4)was used to oxidize sodium hyaluronate(SH)to obtain aldehyde products.Then,the modified cationic silk fibroin(CSF)and oxidized sodium hyaluronate(OSH)were blended to form dynamic imine bond through Schiff base reaction,which made the hydrogel formed injectable.Finally,the influence of the volume ratio of CSF to OSH on the gelling time,morphology,chemical structure,mechanical properties and rheological properties of hydrogels was systematically studied.The results show that when the volume ratio of CSF to OSH is 1∶2,the hydrogel exhibits a short gelation time(148±9)s,the maximum storage modulus(354 Pa),a uniform pore structure,and favorable injectability.This system does not require chemical cross-linking agents and provides a theoretical reference for the application of SF injectable hydrogels in the biomedical field.