基于网络药理学和实验验证探讨高尤-7味丸改善急性肾损伤的作用机制OA

Objective:To explore the renal protective effects and mechanisms of Gaoyou-7 Pill against acute kidney injury(AKI).Methods:Network pharmacology was employed to identify active ingredients and potential targets of Gaoyou-7 Pill,fol-lowed by PPI network analysis.Common targets of Gaoyou-7 Pill and AKI were subjected to GO and KEGG enrichment analyses using R software.A core component-potential target-disease-signaling pathway network was constructed,and molecular docking was performed between core components and key targets.A LPS-induced AKI mouse model was established to preliminarily eval-uate disease pathology and therapeutic efficacy.Renal histopathological changes were observed via H&E staining,and renal func-tion was assessed using enzymatic assay kits.qRT-PCR was utilized to measure mRNA levels of TNF-α,IL-6,and IL-1β,while Western blot was performed to detect protein expression of PI3K,Akt,and p-Akt.Results:A total of 75 active ingredients of Gaoyou-7 Pill were identified,with 54 potential targets and 7 core components(quercetin,T-muurolol,hexadecanoic acid,alpha caryophyllene,linoleic acid,cis cinnamaldehyde,phenethyl acetate)and 6 core targets(AKT1,TNF,IL6,TP53,IL1B,EG-FR)screened.GO and KEGG analysis revealed significant enrichment in transcription factor activity,cell differentiation,and sig-naling pathways including PI3K/Akt,IL-17,TNF,and HIF-1.In vivo experiments demonstrated that Gaoyou-7 Pill protected renal function,reduced the levels of TNF-α,IL-6,and IL-1β,and upregulated the expression of PI3K and p-Akt/Akt.Conclu-sion:Gaoyou-7 Pill ameliorates AKI through multi-component,multi-target,and multi-pathway mechanisms,primarily by modu-lating the PI3K/Akt pathway and suppressing inflammatory cytokine production,thereby preserving renal function.These findings provide a theoretical foundation for the rational clinical application and further development of Mongolian medicine.