六味地黄丸调控星形胶质细胞A1/A2极化促进髓鞘再生的作用及机制研究OA

Objective:To explore the efficacy and mechanism of Liuwei Dihuang Pills in promoting remyelination in multiple sclerosis.Methods:A total of sixty C57BL/6J mice were randomly divided into the normal control group,the model group,the Fingolimod group and the Liuwei Dihuang Pill low-dose,medium-dose and high-dose groups,with 10 mice in each group.The mice in the model group and the drug group were fed with a diet containing 0.2%dicyclohexanone oxalyl dihydrazone for 5 weeks to induce demyelination.From the 6th week onwards,the normal diet was resumed.Meanwhile,the mice in the treatment group were given intragastric administration of the drug until the end of the 8th week when samples were collected.The luxol fast blue staining observation myelinated area of change;Immunohistochemical determination of the expression of myelin basic protein(MBP),observe the myelin regeneration;Immunofluorescence determination of astrocyte A1/A2 polarization markers,observe the party works for the regulation of A1/A2 polarization;ELISA method to detect the corresponding cytokine IL-1α and TNF-α.Western blot was used to detect the expression of MBP,C3,S100A10,p65 and p-p65 proteins in mice brain tissues.Results:Af-ter gavage administration of Liuwei Dihuang Pill,the myelin sheath LFB staining and MBP expression of mouse brain tissue in-creased(P<0.01).The expressions of key proteins p65 and p-p65 and related cytokines TNF-α and IL-1α in the NF-κB signaling pathway were decreased(P<0.05).AST polarization towards A1 is reduced,and C3 expression is reduced.The polarization of AST in the direction of A2 increased,and the expression of S100A10 increased.Conclusion:Liuwei Dihuang Pill may significant-ly alleviate neuroinflammation,mediate A1/A2 polarization,and promote remyelination in multiple sclerosis by regulating the NF-κB signaling pathway and related cytokines.