拉曼单细胞技术在耐冷硝化菌筛选中的应用OA

As a new type of microbial analysis method,Raman single-cell technology has shown unique advantages in the screening and identification of cold-resistant nitrifying bacteria.This technology realizes non-destructive detection of single cells through Raman spectral characteristics,and can achieve accurate separation of target strains in combination with sorting modules.This study finds that Raman spectroscopy can accurately reflect the metabolic characteristics and activity changes of nitrifying bacteria in low-temperature environments,providing a reliable basis for the rapid screening of cold-resistant strains.By establishing a Raman spectral fingerprint library,the rapid identification and classification of cold-resistant nitrifying bacteria can be achieved.This method significantly improves the screening efficiency of cold-resistant nitrifying bacteria and provides technical support for the optimization of low-temperature biological denitrification processes.