对萼猕猴桃HMGR基因家族的鉴定及其对淹水胁迫的响应OA

3-Hydroxy-3-methylglutaryl coenzyme A reductase(HMGR)is a key enzyme in the terpenoid biosynthetic pathway and plays a vital role in plant growth,development,and responses to abiotic stress.To investigate the characteristics of the AvHMGR gene family and its function under waterlogging stress,a genome-wide identification of the AvHMGR gene family in Actinidia valvata was performed using bioinformatics approaches.Comprehensive analyses were conducted on their chromosomal localization,gene structures,conserved protein domains,and cis-acting elements in promoter regions,etc.The results were as follows:(1)A total of 12 AvHMGR family members were identified in the A.valvata genome and were located across 12 different chromosomes.These AvHMGR genes encoded proteins ranging from 514 to 597 amino acids in length,all of which were predicted to be localized in the endoplasmic reticulum.Based on the phylogenetic analysis,AvHMGR members were grouped into three clusters.(2)Promoter analysis revealed abundant hormone-responsive elements and stress-responsive elements within the AvHMGR promoters.(3)Expression profiling showed that AvHMGR6b and AvHMGR9a/b exhibiting relatively high expression levels at different tissues and fruit developmental stages;AvHMGR5a/b were up-regulated after 12 h of salt stress,suggesting their involvement in short-term salt stress response.(4)Under waterlogging stress,multiple AvHMGR genes including AvHMGR5b,AvHMGR6a/b,AvHMGR9a/b,and AvHMGR21a were significantly up-regulated in both leaves and roots,suggesting that AvHMGRs played a critical role in plant responses to waterlogging stress.The results of this study provide a theoretical foundation for further research on the functional characterization of HMGR in plant responses to waterlogging stress and future breeding effort aimed at enhancing kiwifruit tolerance to waterlogging stress.