近距光学检测在微藻密度测量中的研究进展OA

Indirect microalgae density measurement methods based on short-range optical detection have increasingly attracted attention in the monitoring of marine microalgae cultivation,owing to their advantag-es of non-contact,non-invasive operation and the absence of reagent requirements.This review systemati-cally summarizes prevalent cell measurement strategies,encompassing benchmark methods(manual counting and dry cell weight,DCW)and indirect optical approaches(optical sensing and image analysis).The key characteristics and applicable conditions of each method are analyzed from four perspectives:de-tection range,accuracy,environmental interference,and equipment dependence.In light of existing limi-tations,the establishment of a standardized testing platform covering representative marine microalgae spe-cies,gradient cell densities,and diverse illumination and imaging conditions is proposed,together with a multidimensional,quantifiable,and reproducible evaluation framework.This work is expected to provide systematic references and technical guidance for the selection of microalgae bioprocess monitoring sys-tems,algorithm optimization,and the development of novel sensing paradigms.