活化血小板对内皮细胞凋亡损伤的作用研究OA

Objective To investigate the effect of platelet activation on the apoptosis of endo-thelial cells.Methods Platelets were prepared from venous blood,obtained from healthy sub-jects.Resting platelet suspension,activating platelet suspension,and endothelial cells were cul-tured in vitro to establish the co-culture model of platelets and endothelial cells.The aggregation,adhesion and release process of platelets after activation and the apoptosis process of endothelial cells were observed by live-cell imaging technique.The endothelial cell survival activity and mitochondrial membrane potential were then detected by kits.Results Activated platelets exhibited significant ag-gregation,adhesion,and release responses.Compared to resting platelets,activated platelets ag-gregated,migrated toward endothelial cells,and adhered to them.Live-cell imaging revealed that activated platelets induced nuclear pyknosis,condensation,marginalization,reduced nuclear vol-ume,fragmentation,and the formation of apoptotic bodies in endothelial cells.Compared to the resting platelet group,the activated platelet group showed reduced endothelial cell viability and mi-tochondrial membrane potential.Conclusion Platelet activation induces apoptotic injury in endothe-lial cells.