基于网络药理学和实验验证探究疏清颗粒治疗甲型H1N1流感的作用机制OA

Objective To explore the potential targets and mechanisms of Shuqing Granules in the treatment of influenza A(H1N1).Methods The active components and targets of Shuqing Granules were screened through the TCMSP,and the targets relat-ed to influenza A were obtained in combination with the TTD.Kyoto Encyclopedia of Genes and Genomes pathway analysis was conduc-ted on overlapping targets.A cellular model of influenza A(H1N1)virus infection was established using A549 cells,and the effect of Shuqing Granules on cell viability and anti-infection was detected by the CCK8 method.The content of interferon(IFN)secreted after Shuqing Granules intervention was detected by ELISA.The phosphorylation levels of mitogen-activated protein kinase(MAPK)path-way proteins and the mRNA expression of interferon-stimulated genes(ISGs)were detected by Western blot and real-time quantitative polymerase chain reaction.Results A total of 89 active components of Shuqing Granules and 10 influenza-related target genes were i-dentified.MAPK signaling pathway was the core anti-influenza pathway of Shuqing Granules.Shuqing Granules showed an optimal an-ti-influenza effect at 25.78 mg/mL for 48 h(therapeutic index was 4.51).Shuqing Granules could reduce the phosphorylation levels of extracellular signalregulated kinase and p38 proteins,promote the release of IFN-β,and up-regulated the mRNA expressions of PKR,MxA and OAS1 in ISGs.Conclusion Shuqing Granules exert a significant inhibitory effect against influenza A(H1N1).The mechanism may be related to regulating the phosphorylation of MAPK pathway proteins,inducing IFN-β secretion and up-regulating the expression of downstream ISGs.