菟丝子醇提物自组装纳米粒制备及其改善多囊卵巢综合征的药效评价OA

Objective:To construct Cuscuta chinensis alcohol extract self-assembled nanoparticles(CSE-NPs),analyzing their self-assembly molecular mechanism,and clarifying their therapeutic potential for polycystic ovary syndrome(PCOS).Methods:CSE-NPs were prepared using the solvent diffusion-self-assembly method and systematically characterized.Molecular docking was employed to simulate interactions among the main active components of Cuscuta chinensis to analyze the formation mechanism of the self-assembled nanostructure.A PCOS mouse model was established by letrozole combined with a high-fat diet.Mice successfully modelled were randomly divided into the model group,positive control group(metformin hydrochloride),low-dose CSE-NPs treatment group,and high-dose CSE-NPs treatment group.In order to evaluate the in vivo efficacy of CSE-NPs,one-way ANOVA was used to compare differences among groups in body weight,estrous cycle,plasma testosterone and estradiol levels,behavior and ovarian histopathology.Results:CSE-NPs with a uniform particle size of(164.200±4.371)nm,a ζ-potential of(-27.910±1.156)mV,and stable properties were successfully prepared.Molecular docking revealed that key flavonoid components in Cuscuta chinensis,such as hyperoside,kaempferol and astragalin,can spontaneously assemble with isochlorogenic acid C and quinic acid through hydrogen bonds and π-π stacking to form stable nanostructures.Animal experiments demonstrated that,compared with the model group,High-dose CSE-NPs group significantly ameliorated abnormal body weight gain(P<0.05),restored regular estrous cycles,reduced elevated testosterone levels(P<0.01),increased estradiol levels(P<0.05),improved behavioral indicators,significantly reduced the number of ovarian follicles(P<0.01)and increased the number of corpora lutea(P<0.01).Conclusion:Cuscuta chinensis alcohol extract can successfully self-assemble into stable nanoparticles through molecular interactions between its main active components.CSE-NPs can reduce high androgen levels,improve polycystic ovarian-like lesions,effectively restore the estrous cycle,and intervene in the core pathological aspects of PCOS.This study provides a new strategy and theoretical foundation for the modern development and clinical application of Cuscuta chinensis.