初步探究雌激素对卵巢切除小鼠焦虑样行为、空间探索能力及运动功能的影响OA

Objective:To investigate the effects of estrogen deficiency on anxiety-like behavior,spatial exploration ability,and motor function in mice,and to evaluate the ameliorative effects of exogenous estradiol(E2)intervention.Methods:Thirty healthy adult female Kunming mice(selected from an initial 45 mice weighing 26-30 g after weight screening and preliminary behavioral tests)were randomly divided into three groups:the Sham group(n=6),the OVX group(n=12),and the OVX+E2 treatment group(n=12),with respective surgical procedures performed.The OVX group underwent bilateral ovariectomy;the OVX+E2 group also underwent bilateral ovariectomy and received daily subcutaneous injections of estradiol starting from 3 weeks postoperatively;the Sham group only had ovarian exposure without resection.After 2 weeks of intervention,the elevated plus maze and open field tests were used to evaluate anxiety-like behavior,spatial exploration ability,and motor function in the mice.Results:⑴ Elevated Cross-Maze Experiment:Compared with the Sham group,the number of times and duration of mice entering the open arm decreased and the number of times and duration of staying in the closed arm in the OVX group increased,and the differences were statistically significant(P<0.05);compared with the OVX group,the number of times and duration of mice entering the open arm increased and the number of times and duration of staying in the closed arm decreased in the OVX+E2 group,and the differences were statistically significant(P<0.05).⑵ Open Field Experiment:compared with the Sham group,the number of times and duration of mice entering the central area significantly decreased,and the number of times and duration of staying in the peripheral area increased in the OVX group,while the body extension behavior decreased and the curling behavior increased,and the differences were statistically significant(P<0.05);compared with the OVX group,the number of times and duration of mice entering the central area significantly increased and the number of times and duration of staying in the peripheral area significantly decreased in the OVX+E2 treatment group,while the body extension behavior increased and the curling behavior decreased,and the differences were statistically significant(P<0.05).Compared with the Sham group,the average speed and total movement distance of mice entering the central area in the OVX group decreased,and the differences were statistically significant(P<0.05);compared with the OVX group,the average speed and total movement distance of mice entering the central area in the OVX+E2 group significantly increased,and the differences were statistically significant(P<0.05).Conclusion:Estrogen deficiency leads to increased anxiety-like behavior,decreased spatial exploration ability,and reduced motor function in mice.Exogenous E2 supplementation significantly improves the above cognitive and behavioral abnormalities,suggesting that estrogen has potential protective effects against menopause-related neurological disorders.