秋水仙素对天贵卷瓣兰多倍体的诱导效应OA

[Purpose]Colchicine was used to induce polyploidy in Bulbophyllum tianguii,providing reliable techniques and meth-ods for germplasm innovation and genetic breeding.[Method]Different concentrations of colchicine were used as mutagens.Clus-tered buds of in vitro B.tianguii plantlets were treated via the soaking and mixed-culture methods.The mutagenic effects were sys-tematically evaluated through flow cytometry,morphological observation,and root-tip cell chromosome counting.[Result]Numer-ous chimeras were induced through both the soaking and mixed-culture methods.The soaking method yielded more tetraploids.The treatment with the highest induction rate(6.67%)was soaking in 150 mg·L-1 colchicine for 12 h,while it showed a chimera rate of 38.33%and a mortality rate of 36.67%.Successfully induced polyploid plants showed significant differences in DNA fluorescence intensity absorption peaks compared with diploids.In terms of leaf and stomatal morphology,tetraploid plants had darker green leav-es,longer and wider leaves,and larger gurd cells(both in length and width)compared with diploid plants.However,their stomatal density(14 per field of view)was significantly lower than that of diploid plants(24 per field of view).Root-tip cell chromosome counting showed that the chromosome number was 2n=2x≈44 in diploid plants and 2n=4x≈88 in tetraploid plants.[Conclusion]Colchicine can successfully induce polyploidy in B.tianguii.The induction effects are influenced by colchicine concentration and treatment duration.The polyploid plants exhibit superior phenotypic traits compared with diploids.