基于成簇规律间隔短回文重复序列及其相关蛋白系统辅助分子自组装策略的肿瘤标志物检测研究进展OA

The ultra-low abundance of tumor markers and interference from complex matrices in liquid biopsy represent the primary bottlenecks for early cancer screening,and single amplification strategyis can bardly achieve a good balance among sensitivity,specificity,and reaction speed.In recent years,clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)system-assisted molecular self-assembly strategy was used in detection of tumor biomarker.Leveraging the precise recognition and efficient cleavage activity of CRISPR-Cas system,this synergistic strategy effectively overcomes the challenges of background leakage and sluggish kinetics associated with traditional enzyme-free self-assembly.By constructing a cascade signal amplification loop,CRISPR-Cas system-assisted molecular self-assembly strategies enable the ultrasensitive and highly specific detection of trace markers such as circular tumor DNA(ctDNA)and micro RNA(miRNA).Herein,recent research progress on CRISPR-Cas system-assisted molecular self-assembly strategies for tumor marker detection was reviewed.The applications of this dual signal amplification mechanism in the field of physical sensing were introduced.The deep integration of the CRISPR-Cas system-assisted molecular self-assembly strategies with artificial intelligence,solid-state reagent storage,and microfluidic technology constituted a pivotal direction for advancing its application in clinical point-of-care testing.