中国南瓜生长调节因子的基因鉴定及表达分析OA

Growth-regulating factors(GRFs),a type of plant-specific transcription factors,have been demonstrated to be involved in various biological processes,including leaf growth,floral organ development,and phytohormone signal transduction.In this study,GRF gene family members in Cucurbita moschata were identified by using the bioinformatic methods.The physicochemical properties,chromosomal localization,gene structure,evolutionary relationship,collinearity and cis-acting elements within the promoter regions were analyzed.The expression patterns of CmoGRF genes across different tissues were further analyzed in combination with transcriptome analysis.The results showed that a total of 14 CmoGRF genes family members were identified in the pumpkin genome,which were distributed across 11 different chromosomes.Through the phylogenetic analysis,the members of the CmoGRF gene family could be classified into four subgroups.Eleven pairs of syntenic genes were detected within the CmoGRF family,although no tandem duplicated genes were observed.The interspecies collinearity analysis revealed that nine CmoGRF genes had syntenic counterparts in Arabidopsis thaliana,while five CmoGRF genes were homologous to the genes in Oryza sativa.Within the promoters of CmoGRFs,five types of elements related to plant hormone response,four types of metabolic differentiation regulatory elements,four types of protein-binding sites,and three types of cis-acting elements associated with the defense and stress responses were screened.The relative expression levels of the 14 CmoGRFs genes varied significantly across different tissues.The expression pattern analysis of CmoGRF1,CmoGRF12,and CmoGRF13 revealed that,the CmoGRF1 gene was highly expressed under the induction of 6-benzylaminopurine(6BA),methyl jasmonate and zeatin.The CmoGRF12 gene was highly expressed under the induction of methyl jasmonate.The expression of the CmoGRF13 gene was significantly increased under the treatments with abscisic acid and methyl jasmonate.In summary,the results laid a foundation for further analysis on the functional characteristics and molecular mechanisms of the GRF gene family in Cucurbita moschaa,providing a theoretical foundation and new genetic resources for the breeding of pumpkin.