无芒雀麦种质材料萌发期耐旱性综合评价OA

Drought is one of the primary stress factors affecting plant production,and the germination stage is the most vulnerable period in the plant life cycle.In the breeding of drought-resistant Bromus inermis,evaluat-ing drought tolerance during the germination stage is particularly important.This study employed the double-layer filter paper germination method,measuring eight indicators-including germination rate,germination poten-tial,germination index,root length,and shoot length-under 25%PEG-6000 stress for 100 B.inermis germ-plasm resources.Principal component analysis,membership functions,and hierarchical clustering analysis were used to comprehensively assess drought tolerance during the germination stage.The results showed that,com-pared to the control,all observed indicators decreased under 25%PEG stress,with coefficient of variation rang-ing from 20.853%to 97.876%.There was a significant correlation(P<0.05)among most experimental indi-cators.There were substantial differences in drought tolerance among materials,with the comprehensive drought tolerance coefficient ranging from 0.612 to 0.169 and the D-value ranging from 0.861 to 0.068.Clus-ter analysis classified the 100 t100 Bromus inermis germplasm resources into four groups:highly drought-resis-tant(14 materials),moderately drought-resistant(34 materials),weakly drought-resistant(45 materials),and drought-sensitive(7 materials).This study identified drought-resistant germplasm such as W1Z622,W1Z627,and W1Z595.The research provides high-quality materials and theoretical support for drought-resis-tant breeding of B.inermis.