三江源区燕麦与箭筈豌豆混播对根际土壤微生物多样性的影响OA

The Sanjiangyuan region is an important ecological barrier in China,and the diversity of soil microorganisms in this region is critical for sustaining ecosystem stability.This study created artificial mixed sowings of oat(Avena sativa)and common vetch(Vicia sativa)at ratios of 7∶3(Y7J3),5∶5(Y5J5),and 3∶7(Y3J7),with oat monoculture and common vetch monoculture as controls,in order to examine the effects of mixed sowing on soil nutrients and rhizosphere microbial communities.The Mantel test was used to assess the connections between changes in soil nutrients,rhizosphere microbial community structure,and diversity.It was found that mixed sowing considerably raised the soil pH,organic matter,total nitrogen,and available phosphorus content(P<0.05).The Y7J3 treatment showed the greatest improvement(P<0.05).This sowing mix considerably enhanced the relative abundance of Acidobacteriota,RB41 and Pseudomonas in the bacterial community and Ascomycota,Mortierellomycota,Thelebolus and Exophiala in the fungal community(P<0.05).Mantel test analysis revealed that the main elements influencing the control of soil microbial communities were soil pH,organic matter,available nitrogen,and available phosphorus(P<0.05).In conclusion,by altering the inter-root soil nutrients,the mixed sowing of oat and common vetch had a significant impact on the structure and diversity of the soil microbial community.The best results were obtained when the oat and common vetch were sown in 7∶3,and soil pH and organic matter were highly important in the regulation of the microbial community.These results provide a scientific basis for useful recommendations for enhancing soil ecological function and optimizing artificial grassland planting in the Sanjiangyuan region.