柴芩颗粒对动物急性炎症的抗炎作用及机制OA

Objective To investigate the anti-inflammatory effect and mechanisms of Chaiqin Granules on acute inflammation in animals based on a pharmacodynamic evaluation system with three animal models.Methods Three inflammatory models were established,including xylene-induced ear edema in mice,acetic acid-induced increased peritoneal capillary permeability in mice,and carrageenan-induced paw edema in rats,to observe the anti-inflam-matory effect of Chaiqin Granules.Enzyme-linked immunosorbent assay(ELISA)was employed to detect the contents of IL-6,IL-17,IL-1β,IL-18,NLRP3 inflammasome and Caspase 1 in the serum and paw edema tissues of carrageenan-induced inflammatory rats.Results Compared with control group,medium and high-dose groups of Chaiqin Granules both inhibited xylene-induced ear edema in mice(P<0.01,P<0.05).Medium-dose group of Chaiqin Granules significantly suppressed acetic acid-induced increase in peritoneal capillary permeability in mice(P<0.05).High,medium and low-dose groups of Chaiqin Granules reduced the contents of inflammatory factors IL-6,IL-17 and IL-1β in rat serum to varying degrees(P<0.05,P<0.01,P<0.001),and simultaneously decreased the contents of IL-18,IL-1β,Caspase 1 and NLRP3 in paw tissues(P<0.05,P<0.01,P<0.001).Conclusion Chaiqin Granules have an inhibitory effect on acute inflammation.They can alleviate inflammatory responses by inhibiting the NLRP3 inflammasome signaling pathway,regulating the release of inflammatory factors IL-1β and IL-18,and reducing the levels of inflammatory factors IL-6,IL-17 and IL-1β in serum.