五味子酚对小鼠肝癌H22细胞移植瘤生长的影响OA

Objective To investigate the effect of schisanhenol(SAL)on the growth of transplanted H22 hepa-toma cells in mice.Methods A mouse model of transplanted H22 hepatoma cells was established.After the formation of transplanted tumors,15 C57BL/6 mice were divided into model group,SAL 10 mg/kg group,and SAL 20 mg/kg group,with 5 mice in each group.The volume of transplanted tumors in mice was measured using a vernier caliper,and the tumor inhibition rate was calculated.Hematoxylin-eosin staining and immunohistochemical staining were used to detect the effects of SAL on the histomorphology of transplanted H22 hepatoma tissues and the expression of Ki-67 protein in transplanted tumor tissues,respectively.Western blot was employed to determine the expression levels of p-STAT3(Tyr705),Bcl-2,and Cyclin D1 proteins in mice transplanted tumor tissues.Results Compared with model group,both SAL 10 and 20 mg/kg groups significantly inhibited the volume of transplanted H22 hepatoma cells in mice(P<0.05)in a dose-dependent manner.The results of immunohisto-chemical staining showed that compared with model group,both SAL 10 and 20 mg/kg groups could inhibit the expression of Ki-67 protein in the transplanted H22 hepatoma tissues of mice.Western blot results demonstrated that compared with model group,both SAL 10 and 20 mg/kg groups significantly inhibited the expression of p-STAT3(Tyr705),Bcl-2,and Cyclin D1 proteins(P<0.01)in a dose-dependent manner.Conclusion Schisan-henol inhibits the growth of transplanted H22 hepatoma cells in mice,and its mechanism may be related to the in-hibition of p-STAT3(Tyr705)and the expression of downstream apoptotic gene Bcl-2,as well as the promotion of Cyclin D1 expression.