不同产地生地黄主要成分与抗氧化活性评价OA

To investigate the differences in main chemical components and in vitro antioxidant activity of Rehmannia glutinosa Libosch.from different geographical origins,and to provide a basis for the quality evaluation and rational utiliza-tion of its medicinal resources.The contents of catalpol,rehmannioside D,verbascoside,and echinacoside in 16 batches of Rehmannia glutinosa Libosch.were determined by HPLC-PDA.The antioxidant activities were evaluated by DPPH and ABTS free radical scavenging assays.The relationship between chemical components and antioxidant activity was ex-plored by combining cluster analysis(HCA),principal component analysis(PCA),and Pearson correlation analysis.Re-sults showed that there were significant differences in the chemical composition of samples from different origins.The highest content of catalpol(31.021 mg/g)was found in samples introduced from Linfen,Shanxi to Xinjiang.The high-est content of rehmannioside D(5.633 mg/g)was in samples from Jiaozuo,Henan.The highest contents of echinacoside(0.509 mg/g)and verbascoside(1.352 mg/g)were in samples introduced from Quwo County and Baoyan,Shanxi to Xinjiang,respectively.Samples introduced from Quwo County,Shanxi to Xinjiang exhibited the strongest antioxidant ac-tivity(DPPH IC50 8.97 mg/mL,ABTS IC50 5.29 mg/mL).Correlation analysis indicated that the content of echinaco-side was significantly negatively correlated with the DPPH IC50 value(P<0.05),suggesting it as a key antioxidant com-ponent.In summary,the introduced Rehmannia glutinosa Libosch.in Xinjiang exhibits unique advantages in terms of specific chemical component accumulation and comprehensive antioxidant activity.This study provides a scientific basis for the quality evaluation,origin selection,and identification of key quality markers of Rehmannia glutinosa Libosch.