基于线粒体16S rRNA基因序列的3个雌核发育锦鲤品系的遗传差异分析OA

Based on the mitochondrial 16S rRNA gene sequence,this study analyzed the genetic diversity of 3 Cyprinus carpio varieties(pure red,pure white,and pure black)cultivated by gynogenesis using the GenBank database and MEGA 5.0 software.The results showed that the length of PCR amplification products ranged from 750 to 1 000 bp,which met the requirements for sequencing.Sequencing analysis indicated that the length of the extracted 16S rRNA gene fragment was 967 bp,with an average A+T content of 58.26%,which was higher than the G+C content(41.74%),consistent with the A and T bias characteristics of fish mitochondrial genes.Phylogenetic analysis showed that the genetic distances between Cyprinus carpio Gyno Red and Cyprinus carpio Gyno Black,Cyprinus carpio Gyno Black and Cyprinus carpio Gyno White,and Cyprinus carpio Gyno White and Cyprinus carpio Gyno Red were 0.10%,4.80%,and 5.00%,respectively;Cyprinus carpio Gyno Black and Cyprinus carpio Gyno Red had a close genetic relationship,while Cyprinus carpio Gyno White clustered independently into one branch.The research results provide references for the directional selection,genetic improvement of high quality body color strains and population evolution research of Cyprinus carpio.