山仙颗粒含药血清通过Wnt/β-catenin信号通路抑制肝卵圆细胞EMT及恶性转化相关表型OA
Shanxian granule-containing serum inhibits epithelial-mesenchymal transition(EMT)and malignant transformation of hepatic oval cells via the Wnt/β-catenin signaling pathway
目的:本研究旨在探讨山仙颗粒(SXG)含药血清通过Wnt/β-连环蛋白(β-catenin)信号通路逆转肝卵圆细胞系WB-F344上皮间质转化(EMT)及恶性转化相关表型的作用机制.方法:采用N-甲基-N'-硝基-N-亚硝基胍(MNNG)联合过氧化氢(H2O2)诱导WB-F344细胞EMT及恶性转化模型.实验分为对照组(Control)、模型组(MNNG)、SXG低/中/高剂量组(SXG-L、SXG-M、SXG-H)及通路验证组(SXG-H+Wnt激活剂CP21R7).通过MTT法检测细胞增殖活性,划痕试验及Transwell实验评估细胞迁移及侵袭能力.Western Blot检测EMT标志蛋白(E-cadherin、N-cadherin、Vimentin)、恶性转化相关蛋白(cleaved Caspase-3、P53、CD34)及 Wnt 通路关键分子(Wnt3a、β-catenin、C-myc)的表达.结果:与对照组相比,模型组细胞增殖、迁移及侵袭能力显著增强(P＜0.05),E-cadherin、cleaved Caspase-3、P53 蛋白表达下调,N-cadherin、Vimentin、CD34 及 Wnt 通路相关蛋白表达上调(P＜0.05);SXG 含药血清干预后,上述恶性表型及EMT进程呈剂量依赖性逆转,且CP21R7可部分拮抗SXG的上述效应(P＜0.05).结论:SXG可通过抑制Wnt/β-catenin信号通路,阻断WB-F344细胞EMT及恶性转化相关表型.
Objective:This study aimed to investigate the mechanism by which Shanxian Granule medicated serum(SXG)reverses epithelial-mesenchymal transition(EMT)and malignant transformation of the hepatic oval cell line WB-F344 through the Wnt/β-catenin signaling pathway.Methods:The malignant transformation and EMT model were induced in WB-F344 cells using N-methyl-N'-nitro-N-nitrosoguanidine(MNNG).Experimental groups were treated with low-,medium-,and high-dose SXG,while control groups included a normal group(blank serum),a model group(MNNG+blank serum),and a pathway validation group(high-dose SXG+Wnt activator CP21R7).Cell proliferation was assessed via MTT assay;migration and invasion abilities were evaluated using scratch wound healing and Transwell assays.Western blotting was employed to detect EMT-related markers(E-cadherin,N-cadherin,Vimentin),malignant transformation-associated proteins(cleaved Caspase-3,P53,CD34),and key molecules of the Wnt pathway(Wnt3a,β-catenin,C-myc).Results:Compared with the normal group,the model group exhibited significantly enhanced cell proliferation,migration,and invasion(P＜0.05),accompanied by downregulated E-cadherin,cleaved Caspase-3,and P53 protein levels,and upregulated N-cadherin,Vimentin,CD34,Wnt3a,β-catenin,and C-myc expression(P＜0.05).SXG intervention reversed these malignant phenotypes and EMT progression in a dose-dependent manner.However,the addition of CP21R7 antagonized the effects of SXG(P＜0.05).Conclusion:SXG inhibits EMT and malignant transformation of WB-F344 cells by suppressing the Wnt/β-catenin signaling pathway.
屈梦扬;潘艳芳;贾晓涛;杨雅倩;杨鑫茂;王院春;齐宝宁
陕西中医药大学基础医学院(陕西 咸阳,7120462)陕西中医药大学基础医学院(陕西 咸阳,7120462)西安市中心医院神经内科陕西中医药大学基础医学院(陕西 咸阳,7120462)陕西中医药大学基础医学院(陕西 咸阳,7120462)陕西中医药大学附属医院陕西中医药大学基础医学院(陕西 咸阳,7120462)
医药卫生
山仙颗粒上皮间质转化恶性表型癌前病变肝卵圆细胞Wnt/β-catenin信号通路
Shanxian granulecontaining serumepithelial mesenchymal transitionmalignant transformationprecancerous lesions of liverhepatic oval cellsWnt/β-catenin signaling pathway
《中西医结合肝病杂志》 2026 (4)
451-455,5
国家自然科学基金项目(No.81703842),陕西省重点研发计划项目(No.2024SF-YBXM-147),咸阳市重点研发计划(No.L2025-ZDYF-JBFZ-017)
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