小麦条锈菌生理小种CYR34中GST基因家族的鉴定、结构特征及表达OA

To clarify the detoxification metabolism and environmental adaptation mechanisms of Puc-cinia striiformis f.sp.tritici(Pst)physiological race CYR34,members of the glutathione S-transferase(GST)gene family were identified based on the their nucleotide sequence and GFF3 annotation files of CYR34.Bioinformatics analyses were conducted on the physicochemical properties of the encoded pro-teins,chromosomal localization,phylogenetic relationships,and cis-acting elements in the promoter regions.In addition,the temporal expression patterns of GST family genes during urediniospore germi-nation and host infection were analyzed by quantitative real-time PCR.The results showed that six GST genes(GST1-GST6)were identified in CYR34.The encoded proteins ranged from 187 to 207 amino acids in length,all containing conserved GST domains and being distributed on three chromosomes.A total of 26 types of cis-acting elements were detected in the promoter regions.During urediniospore ger-mination,GST1,GST3,GST4,GST5,and GST6 all reached relatively high expression levels at 24 hours post inoculation(hpi),among which GST5 showed the greatest up-regulation,reaching 9.04-fold that of the control group.During wheat infection by CYR34,GST4 and GST6 generally exhibited an upward expression trend,with GST4 reaching its highest expression level at 48 hpi,3.14-fold that of the control group.These results indicate that the GST genes in CYR34 may participate in the regulation of environ-mental adaptation and detoxification metabolism during urediniospore germination and infection.