授粉蜂群人工培育蜂王技术研究OA

To address the decline in pollination efficiency caused by limited foraging and difficulties in queen replacement under the low-temperature,low-light conditions typical of winter-spring greenhouse strawberry production,this study proposes and validates an indoor ex nido artificial rearing and application protocol for pollination oriented functional queens.We established a standardized queen-rearing workflow under controlled temperature,humidity,and low-light conditions;implemented a staged larval transfer with matched well sizes(48 well→24 well→12 well);and compared artificial diets for their effects on developmental quality.Key evaluation metrics included foraging frequency,flower-visit duration,pollen-loading rate,and in-hive feeding order.A diet comprising 65%royal jelly and 35%sterile water yielded morphologically complete eclosed queens suitable for subsequent deployment.The staged transfer strategy aligned phase-specific requirements for space,nutrition,and pupal quiescence,significantly improving pupation integrity and eclosion quality.Following the introduction of artificially eclosed queens into queenless colonies,social order was rapidly restored.During strawberry bloom,workers exhibited stable foraging,adequate pollen loading,and effective stigma contact,with normal feeding and brood care and steady colony strength.This technique enables queen availability in winter,reduces dependence on traditional breeding conditions,provides a viable pathway to stable fruit set and improved marketability in greenhouse strawberries,and offers practical guidance for the standardized supply and management of pollination colonies in protected cultivation.