与传染性支气管炎病毒核衣壳蛋白互作的宿主RNA结合蛋白的鉴定OA

To screen host RNA-binding proteins interacting with the infectious bronchitis virus(IBV)N protein,providing a basis for elucidating the virus-host interaction mechanism,co-immunoprecipitation(Co-IP)combined with mass spectrometry was employed to identify host proteins interacting with the N protein with the monoclonal anti-body 4H4 against IBV N protein.Differentially expressed proteins were subjected to bioinformatics analysis,and Co-IP experiments were conducted to validate the interactions between the N protein and candidate RNA-binding pro-teins.The results showed that a total of 847 differentially expressed proteins were identified,primarily involved in processes such as transcription,translation and protein modification,protein-RNA complex assembly,signal trans-duction,and energy metabolism.Co-IP experimental results indicated that interactions exist between the N protein and the host RNA-binding proteins TARDBP,hnRNPK,FUBP3,and hnRNPA1.This study identified host proteins interacting with the IBV N protein,which are involved in multiple key stages of the viral replication cycle.The inter-actions between the N protein and RNA-binding proteins provide new research directions for elucidating the specific mechanisms of host proteins in viral infection.