环状多肽OCP 2对高原缺氧小鼠脑组织保护作用OA

Aim To clarify the anti-high-altitude hy-poxia activity of OCP 2 and its protective effect on brain tissue of high-altitude hypoxia mice.Method The survival time of mice under atmospheric pressure and hypoxia at different dosing intervals and doses were observed.Sixty mice were randomly divided into a blank control group,an hypoxia model group,an ac-etazolamide group,and OCP 2 low-,medium-,and high-dose groups,with 10 mice in each group.The blank control group was placed in a barrier environ-ment,while the remaining groups were placed at a simulated altitude of 8000 m.After 24 hours,patho-logical analysis of brain tissue was performed to deter-mine hypoxia related indicators.Results The optimal dosing interval was 10 minutes,and OCP 2(5-100 mg·kg-1)significantly improved the survival time of mice under atmospheric pressure and hypoxia.Com-pared with the blank control group,the pathological score of the hypoxia model group significantly in-creased.The levels of catalase(CAT),superoxide dismutase(SOD),and glutathione(GSH)were sig-nificantly reduced,and the expression levels of peroxi-some proliferators activated receptors-γ(PPAR γ)and its co activator 1 alpha(PGC1 α)were significantly re-duced.The levels of inflammatory factors such as in-terleukin-6(IL-6),malondialdehyde(MDA),and hy-poxia inducible factor 1 alpha(HIF-1 alpha)signifi-cantly increased.Compared with the hypoxia model group,the pathological scores of each OCP2 group sig-nificantly decreased.The levels of SOD,CAT,and GSH,as well as the expression levels of PGC-1 α and PPAR γ,significantly increased,while the levels of in-flammatory factors such as IL-6,MDA,and HIF-1 α expression significantly decreased.Conclusion OCP 2 has a good protective effect on brain injury in mice with high altitude hypoxia,which may be related to im-proving antioxidant capacity,inhibiting inflammatory factors and HIF-1α/PGC1α/PPARγ pathway.