自选小麦新品种(系)的高分子量麦谷蛋白亚基组成分析OA

High molecular weight wheat glutenin subunits(HMW-GS)are an important factor affect-ing wheat(Triticum aestivum L.)quality,and the analysis and selection of their composition is an important part of the research for the selection and breeding of new high-quality varieties.In this study,42 new wheat varieties(lines)were independently selected by the group as materials,and their HMW-GS composition and quality-related traits were identified based on comprehensive agronomic i-dentification,sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE),and molecu-lar markers.The results showed that a total of 9 subunit types of HMW-GS appeared in 42 new wheat lines.3 subunit types,1,N and 2*,were found at Glu-A1 locus with frequencies of 86.04％,11.63％and 2.33％,respectively;4 subunit types,7,7+8,7+9 and 6+8,appeared at Glu-B1 locus with frequencies of 6.98％,41.86％,48.84％and 2.33％;At Glu-D1 locus,the subunit type of 2+12 ap-peared with frequency was 35.71％;The frequency of high quality subunit 5+10 appeared as high as 64.29％.There were 9 types of subunit combinations,with the highest frequency of 33.33％for the 1/7+9/5+10 combination;23.81％for the 1/7+8/5+10 combination;11.90％for the 1/7+8/2+12 combination;and the frequencies of the other 6 combinations were all below 10％.The results of qual-ity scoring showed that the self-selected lines scored 5-10 points,with an average score of 8 points,and 10 lines scored up to 10 points.Comprehensive agronomic traits were identified to select and breed 3 medium-gluten varieties,KD 456,KD 808,and Kelo No.5.The protein content of 19 lines,inclu-ding Kezi No.1,reached the standard for high-gluten wheat.