非靶向代谢组学分析不同山银花种质的化学成分差异OA

To conduct targeted breeding of high-yield and high-quality mountain Lonicerae Flos varie-ties suitable for the ecological conditions of Suiyang area in Guizhou Province,ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-ESI-MS/MS)and headspace-solid phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)dual-platform non-targeted metabolomics techniques were employed to detect and analyze the metabo-lites of 3 mountain Lonicerae Flos(Lonicera macranthoides)varieties(including Xianglei Lonicera japonica LF1 and 2 local germplasms from Suiyang LF2 and LF3)and 1 Lonicera japonica germ-plasm Jiufeng No.1(LF4).The differences in metabolite composition among the different Lonicerae germplasms were systematically compared.Principal component analysis and orthogonal partial least squares discriminant analysis(OPLS-DA)results showed that the separation trend of metabolites be-tween different varieties was significant.The KEGG pathway enrichment analysis of the differential metabolites indicated that the Lonicera japonica LF4 had higher content of metabolites in the flavonoid bio-synthesis pathway(such as luteolin and quercetin),while the local Lonicerae Flos LF2 and LF3 had higher content of metabolites in the monoterpenoid biosynthesis pathway(such as linalool and menthol).11 differen-tial metabolites common to both Suiyang Lonicerae Flos and Xianglei Lonicera japonica,and 5 differential metabolites common to both Lonicerae Flos and Lonicera japonica were preliminarily screened.These metab-olites could serve as potential markers for the identification of different varieties.The local Lonicerae Flos germplasm(such as LF2)from Suiyang had significant advantages in the content of monoterpenoids,phenolic acids,and saponins.The research results provided important basis for the identification of Lonicerae Flos germplasms and the selection of superior varieties.