高羊茅FabHLH63基因克隆及在干旱胁迫下表达分析OA
Cloning of the FabHLH63 Gene of Festuca elata and Analysis on Its Expression under Drought Stress
为了培育适应性强、抗逆性好的高羊茅新品种,利用RACE克隆技术从高羊茅干旱转录组中筛选并克隆了FabHLH63,进行生物信息学、亚细胞定位及干旱胁迫下表达分析.结果表明,获得FabHLH63基因完整cDNA序列1 311 bp,其编码一个含359个氨基酸残基的蛋白,具有典型的bHLH结构域;系统进化分析发现,与禾本科植物黑麦草(Lolium multiflorum)的bHLH蛋白亲缘关系较近;亚细胞定位显示FabHLH63蛋白定位于细胞核.实时荧光定量PCR结果表明,在干旱胁迫下,FabHLH63的表达显著上调,说明FabHLH63可能在抗旱响应中发挥了重要作用.研究结果为深入理解FabHLH63在高羊茅抗逆中的分子机制提供了理论依据,并为高羊茅分子育种提供了潜在的基因靶点.
In order to cultivate high-yielding and drought-resistant new varieties of Festuca elata,the RACE technology was used to screen and clone FabHLH63 from the drought transcriptome of Festu-ca elata.Bioinformatics,subcellular localization and expression analysis under drought stress were conducted.The results showed that the complete cDNA sequence of the FabHLH63 transcription fac-tor was 1 311 bp,encoding a protein containing 359 amino acid residues,with a typical bHLH do-main.The phylogenetic analysis revealed that it had a closer genetic relationship with the bHLH pro-tein of the grass species Lolium multiflorum.The subcellular localization indicated that the FabHLH63 protein was located in the nucleus.The results of real-time fluorescent quantitative PCR showed that the expression of FabHLH63 was significantly upregulated under drought stress,indica-ting that FabHLH63 could play an important role in the drought response.The research results pro-vided a theoretical basis for in-depth understanding of the molecular mechanism of FabHLH63 in the drought resistance of Festuca elata,and offerred potential gene targets for molecular breeding of Festuca elata.
陈锡;龙显英;朱必达;赵德刚;王小利
贵州省农业科学院,农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州省农作物品种资源研究所,贵州省草业研究所,贵阳 550006贵州省农业科学院,农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州省农作物品种资源研究所,贵州省草业研究所,贵阳 550006贵州省农业科学院,农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州省农作物品种资源研究所,贵州省草业研究所,贵阳 550006贵州省农业科学院,农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州省农作物品种资源研究所,贵州省草业研究所,贵阳 550006贵州省农业科学院,农业农村部喀斯特山区作物基因资源与种质创新重点实验室,贵州省农作物品种资源研究所,贵州省草业研究所,贵阳 550006
农业科技
FabHLH63基因克隆生物信息学亚细胞定位
FabHLH63 genecloningbioinformatics analysissubcellular localization
《种子》 2026 (3)
30-37,8
贵州省农业科学院开放基金项目(黔农科开放基金[2024]01号)、贵州省科技计划项目(黔科合基础-ZK[2022]一般222)贵州省育种科研基础平台创新能力建设(黔科合服企[2022]014)喀斯特山区特色作物生物育种创新能力建设(黔科合服企[2024]003-1)和喀斯特山区特色粮油作物生物育种部省共建重点实验室科研能力条件建设(黔科合服企[2024]003-2)
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