菘蓝果实外果皮对自身种子萌发抑制作用及生理机制研究OA

To explore the mechanism of the effect of the extract from the Isatis tinctoria fruit on the seed ger-mination,based on the water absorption characteristics and viability of the Isatis tinctoria siliques and seeds,the extraction time for the preparation of the Isatis tinctoria fruit extract was determined.Using non-target metabolite detection,water-soluble differential metabolites were screened,and through exogenous application experiments,the effects of this substance and the original extract on the physiological activity of Isatis tincto-ria seeds in the early stage(0-3 days)of germination were analyzed.The results showed that the Isatis tinc-toria siliques soaked for 20 hours reached the state of seed swelling,with the maximum water absorption rate of 291％,while the Isatis tinctoria seeds reached the state of swelling after soaking for 6 hours,with the maximum water absorption rate of 60.5％.The control group had a germination rate of 93.67％,the silique extract group had a germination rate of 6.67％,and the Isatis tinctoria seed extract group had a germination rate of 69.33％.163 differential metabolites with|log2(FC)|≥5 were screened,among which 41 were wa-ter-soluble differential metabolites.Exogenous application of 3-hydroxy-2-pyridinecarboxylic acid had a strong inhibitory effect on Isatis tinctoria seed germination.The exogenous application of the extract and 3-hydroxy-2-pyridinecarboxylic acid solution affected the changes in the contents and activities of starch,SBE,sucrose,sucrosease,POD,soluble protein,GA,IAA and ABA in Isatis tinctoria seeds.The study showed that the Isatis tinctoria silique extract had an inhibitory effect on its own seed germination,and it was the combined effect of multiple substances.The inhibitors were mainly concentrated in the exocarp of the Isatis tinctoria si-lique and the inhibitors regulated seed germination by affecting the metabolism of substances such as starch,soluble protein,sucrosease,SBE and IAA in Isatis tinctoria seeds.