当归多糖对激素性股骨头坏死大鼠骨代谢的影响OA

Objective:To explore the effect of angelica polysaccharide on bone metabolism in rats with hormonal femoral head necrosis based on the AMP-activated protein kinase(AMPK)/mammalian target of rapamycin(mTOR)signaling pathway.Methods:In this study,the rats were divided into control group,model group,L-angelica polysaccharide group,M-angelica polysaccharide group,H-angelica polysaccharide group,and angelica polysaccharide+inhibitor group,with 12 per group.Except for the control group,the remaining 5 groups were injected with lipopolysaccharides and methylpred-nisolone to induce steroid-induced femoral head necrosis in rat models.Micro-CT scans were used to analyze rat bone min-eral density(BMD),bone volume/tissue volume(BV/TV),and trabecular thickness(Tb.Th),and the number of trabe-cules(Tb.N).HE staining was used to observe the pathological morphology of the femur.TRAP staining was performed to identify osteoclasts.Immunohistochemical staining was implemented to explore the expression of bone metabolism-related proteins.DHE staining was used to measure reactive oxygen species(ROS)level.Moreover,Western Blot was implemented to measure the expression of AMPK/mTOR pathway proteins in the femoral tissue of rats.Results:Compared with the control group,the model group showed a decrease in BMD,BV/TV,Tb.Th,and Tb.N of the femoral head in rats,a reduction in cartilage area and trabecular area,and a large number of empty cavities,the number of osteoclasts on the bone surface increased,the numbers of Osterix,Runx related transcription factor 2(RUNX2),and osteocalcin(OCN)positive cells decreased,the ROS level and mTOR protein expression increased,while p-AMPK protein expression decreased(P<0.05).Compared with the model group,the L-angelica polysaccharide group,M-angelica polysaccharide group,and H-angelica polysaccharide group showed an increase in BMD,BV/TV,Tb.Th,and Tb.N of the femoral head in rats,an increase in trabecular area,a decrease in empty cavities,a decrease in the number of osteoclasts on the bone surface,a raise in Osterix,RUNX2,and OCN positive cells,a decrease in ROS level and mTOR protein expression,and a raise in p-AMPK protein expression(P<0.05).Compared with the H-angelica polysaccharide group,the angelica polysaccharide+inhibitor group showed a decrease in BMD,BV/TV,Tb.Th,and Tb.N in the femoral head in rats,a decrease in trabecular area,a raise in empty cavities,an increase in the number of osteoclasts on the bone surface,a decrease in Osterix,RUNX2,and OCN positive cells,an increase in ROS level and mTOR protein expression,and a decrease in p-AMPK protein expression(P<0.05).Conclusion:Angelica polysaccharide enhances bone metabolism in rats with hormonal femoral head necrosis by regulating the AMPK/mTOR signaling pathway.