二倍体马铃薯叶绿体基因组特征及进化分析OA

Using 3 kinds of diploid Solanum tuberosum species,namely Solanum coense,Solanum rodontum and Solanum notomum,as materials,a study on the racteristics and evolutionary relation-ships of the chloroplast genomes of these diploid Solanum tuberosum using high-throughput sequen-cing methods was conducted.The results showed that the chloroplast genomes of all 3 materials had a typical circular structure,with full lengths of 155 529,155 530 bp,and 155 554 bp respectively.Functional genes were annotated to 84,85,and 84 respectively,among which the clpP gene specific to Small Solanum rodontum was absent in the other 2 materials.All 3 genomes contained 8 ribosomal RNAs(rRNA),while the transfer RNAs(tRNA)were 37,35,and 37 respectively.Codon analysis detected 64 codons,among which 32 codons had RSCU values greater than 1,and the RSCU values of GCU,UUA,AGA,and UCU exceeded 1.6.Sequence similarity comparison revealed that the 3 re-gions with significant differences were located at 10 kb,46 kb,and 84 kb of the genome respectively.Co-linearity analysis showed that the IR regions of Solanum coense and Solanum rodontum had con-tracted during evolution compared to Solanum notomum.Symatic evolutionary analysis indicated that Solanum coense and Solanum rodontum were more closely related,while Solanum notomum was more closely related to tetraploid Solanum tuberosum.