基于网络药理学和分子对接探讨黄芪虫藤饮治疗糖尿病周围神经病变作用机制OA

Objective To explore the potential mechanism of Huangqi Chongteng Decoction in treating diabetic peripheral neuropathy(DPN)based on network pharmacology and molecular docking technology.Methods The active components and potential targets of Huangqi Chongteng Decoction were screened through TCMSP and BATMAN 2.0 databases.GeneCards database was retrieved to obtain DPN targets.R language 4.2.3 was used to analyze intersection targets and construct protein interaction network and drug-active component-target-disease network.GO and KEGG pathway enrichment analysis was performed,and target docking was simulated by AutoDockTools 1.5.6.Results A total of 633 active components and 971 targets of Huangqi Chongteng Decoction were obtained,along with 681 disease targets for DPN.Among these,153 drug-disease intersection targets were identified,with IL6,INS,TNF,IL1B,STAT3,ALB,AKT1,EGFR,IL10 and TP53 identified as the core targets.GO functional enrichment analysis revealed that the biological processes mainly included response to oxygen-containing compounds,regulation of biological quality,and response to stress;the cellular components primarily involved extracellular space,extracellular region,vesicles,cytoplasmic vesicles,and the endomembrane system;the molecular functions mainly encompassed protein binding,signaling receptor binding,identical protein binding,and signaling receptor regulator activity.KEGG pathway enrichment primarily involved insulin resistance and the HIF-1 signaling pathway.Molecular docking results demonstrated that quercetin exhibited high binding affinity to both IL6 and INS.Conclusion Huangqi Chongteng Decoction may intervene in DPN process by regulating metabolism and glycosylation damage,microcirculation disturbance and hypoxia related pathways through quercetin,genistein and other components.