基于杏仁核-海马探讨羽调音乐对恐惧记忆消退的作用机制OA

Objective:To investigate the mechanism of Yu tune music on fear memory extinction of conditioned fear mouse.Methods:Twenty-eight C57BL6/J male mice were randomly divided into control group,fear group,exposed group,and music group,7 in each group.A conditional fear model of mice was established using Pavlov conditioned reflex paradigm.The fear and anxiety states of mice were detected through fear conditioning test(FCT),elevated plus maze test(EPM),and Open field test(OFT),respectively.The excitability and functional connectivity of mouse brain neurons were observed through rs fMRI.Results:In terms of behavior,compared with the control group,freezing time(FT)value and FT percentage in FCT were significantly increased in the fear group(P＜0.01),while ot value in EPM and CWT value in OFT were significantly decreased(P＜0.01);Compared with the fear group,the FT value and FT percentage of mice in the music group and the exposed group were significantly lower on the 14th and 21st day of intervention(P＜0.01),the exposed group was better than the music group(P＜0.01),the OT and CWT values of the two groups were significantly higher(P＜0.01),and the music group was better than the exposed group(P＜0.01).The results of fMRI analysis of mice brain showed that ALFF in bilateral amygdala,hippocampus,visual cortex,prefrontal cortex,somatosensory cortex and other regions of mice in fear group increased significantly(P＜0.01);The ALFF of bilateral amygdala,hippocampus,prefrontal cortex and striatum in music group was significantly decreased(P＜0.01);ALFF in the left hypothalamus,amygdala,thalamus and other regions of the mice in the exposed group increased significantly(P＜0.01),while Alff in the right part of the hippocampus decreased significantly(P＜0.01).Functional connectivity analysis showed that compared with the control group,the functional connectivity between amygdala and hippocampus in the fear group was significantly increased(P＜0.01);The functional connection between amygdala and hippocampus in music group was significantly decreased(P＜0.01);The functional connection between somatosensory cortex and amygdala was weakened in the exposed group(P＜0.01).Conclusion:Yu tune music significantly facilitates the extinction of fear memories,with its mechanism may be related to modulating neural circuits in the brain associated with fear emotions.