猪卵母细胞体外成熟的影响因素及调控策略研究进展OA

Porcine oocytes have extensive applications in the field of reproduction.In vitro maturation(IVM)of porcine oocytes enhances their utilization rate,providing critical support for advancing related research and applications.However,the quality of IVM porcine oocytes and their embryonic developmental potential remain relatively low,limiting further application of this technology.Key factors influencing porcine oocytes IVM include ovarian preservation conditions(temperature and preservation time),follicle size,oocyte collection methods,quality of the cumulus-oocyte complexes,culture conditions,maturation culture time,oxidative stress and culture methods,all of which are adjustable.Targeted regulation of these factors can effectively promote IVM of porcine oocytes.Specific strategies include:standardizing operational procedures and optimizing culture conditions,adding growth factors and oocyte-secreted factors to the IVM medium,addition of antioxidants to mitigate oxidative stress damage in oocytes,and adopting novel culture methods such as three-dimensional culture method and two-step maturation method.This article reviews the factors influencing IVM of porcine oocytes and the regulatory strategies promoting IVM of porcine oocytes,which can provide reference for improving the quality of IVM of porcine oocytes.