透脓连理汤对脾虚湿困证溃疡性结肠炎大鼠模型结肠局部损伤及组织病理学的影响OA

Objective To investigate the effects of Tounong Lianli Decoction on colonic local injury and his-topathology in rat models with ulcerative colitis(UC)of spleen deficiency and dampness retention syndrome.Methods The UC rat model was established by combining environmental and dietary interventions with 2,4,6-trinitrobenzenesulfonic acid(TNBS)/ethanol enema.The rats were randomly divided into the blank group,model group,mesalazine(SASP)group,and low-,medium-,and high-dose Tounong Lianli Decoction groups.Rats in the low-,medium-,and high-dose groups were given Tounong Lianli Decoction by gavage at doses of 10.8,21.6,and 43.2 g/kg,respectively.Those in the SASP group received mesalazine by gavage at a dose of 0.5 g/kg.Rats in the blank group and model group were administered 0.9%normal saline by gavage at a dose of 10 mL/kg.After 15 consecutive days of corresponding interventions,morphological observation and scoring were performed.Results 1)Disease Activity Index(DAI)scores:Compared with the blank group,the DAI score of the model group was significantly increased(P<0.05).Compared with the model group,the DAI scores of all Chinese medicinal groups(low-,medium-,and high-dose Tounong Lianli Decoc-tion groups)and the SASP group were significantly decreased(P<0.01),with no statistically significant dif-ferences among these Chinese medicinal groups and the SASP group(P>0.05).2)Morphological observa-tion and scoring of colonic tissue injury:The score of the model group was significantly higher than that of the blank group(P<0.01).The scores of all Chinese medicinal groups and the SASP group were significantly lower than that of the model group(P<0.01),and there were no statistically significant differences among these Chinese medicinal groups and the SASP group(P>0.05).3)Pathological damage degree and scoring of colonic tissues:Compared with the blank group,the pathological score of the model group was significantly increased(P<0.01),accompanied by destruction of colonic mucosal structure,reduction of goblet cells,dam-age and disordered arrangement of crypt structures,and massive exudation and infiltration of inflammatory cells.The pathological morphology of colonic tissues in all Chinese medicinal groups and the SASP group was significantly improved compared with the model group,with decreased scores(P<0.05 or P<0.01),and no statistically significant differences were observed among these Chinese medicinal groups and the SASP group(P>0.05).Conclusion Tounong Lianli Decoction has an anti-inflammatory effect and can promote tissue repair,which can improve colonic local injury and pathological changes in UC rats of spleen deficiency and dampness retention syndrome.No significant differences were found among the different-dose groups,which may be related to the small sample size,and further exploration with large samples,multicenter studies is war-ranted in the future.