生物絮凝剂产生菌的筛选鉴定及在页岩油污水处理中的应用OA
Screening and Identification of a Bioflocculant-Producing Strain and Its Application in Shale-Oil Wastewater Treatment
从油田现场的土壤和二沉池污泥中共筛选出56株细菌,其中12株具有明显的絮凝活性,通过复筛获得一株高效絮凝剂产生菌,经分子生物学鉴定为蜡样芽孢杆菌.为了提高其絮凝活性,在普通发酵培养基的基础上,优化了碳源、氮源、pH值、摇床转速、温度和接种量的条件.单因素实验结果表明,在弱碱性条件下,对页岩油的去除率比PAM对页岩油的去除率提高了8.8%～19.3%,生物絮凝剂对页岩油污水的浊度和含油量具有更好的去除效果.
A total of 56 bacterial strains were isolated from oilfield soil and secondary sedimentation tank sludge,among which 12 showed significant flocculation activity.Through secondary screening,a highly efficient bioflocculant-producing strain was obtained and identified as Bacillus cereus using molecular biological techniques.To enhance its activity,culture conditions-including carbon and nitrogen sources,pH,rotation speed,temperature,and inoculum size-were optimized.Single-factor experimental results demonstrated that under weakly alkaline conditions,the shale oil removal rate of this bioflocculant was 8.8%to 19.3%higher than that of polyacrylamide(PAM).Moreover,the bioflocculant achieved superior removal efficiency for both turbidity and oil content in shale oil wastewater.
刘鑫磊;齐晗兵;张宇新;张晓雪
大庆油田有限责任公司第九采油厂东北石油大学土木建筑工程学院东北石油大学土木建筑工程学院东北石油大学土木建筑工程学院
资源环境
生物絮凝剂蜡样芽孢杆菌页岩油污水含油量絮凝活性培养条件优化
microbial flocculantBacillus cereusshale oil wastewateroil contentflocculating activityoptimization of culture conditions
《油气田环境保护》 2026 (2)
29-34,6
大庆市指导项目《页岩油污水厌氧处理工艺中挥发性脂肪酸光学检测机理研究》(zd-2023-43).
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