桔梗总皂苷肠道菌转化产物的化学成分及其抗药物性肝损伤活性研究OA
Chemical components of intestinal bacterial transformation products from total saponins of Platycodonis Radix and their anti-drug-induced liver injury activity
目的 研究桔梗总皂苷肠道菌转化产物的化学成分及其抗肝损伤活性.方法 采用AB-8大孔吸附树脂、正相硅胶、Sephadex LH-20凝胶柱色谱和半制备高效液相色谱等柱色谱技术进行分离纯化,根据所分化合物理化性质及质谱、核磁共振波谱数据鉴定化合物结构.采用对乙酰氨基酚诱导AML12细胞肝损伤模型评价所分离化合物的抗肝损伤活性.结果 从桔梗总皂苷肠道菌转化产物中分离得到16个化合物,分别鉴定为3-O-β-D-吡喃葡萄糖氧基-2β,3β,16α,23-四羟基齐墩果烷-12-烯-28-酸-28-O-α-L-吡喃鼠李糖基-(1→2)-α-L-吡喃阿拉伯糖苷(1)、远志皂苷D(2)、3-O-β-D-吡喃葡萄糖基桔梗皂苷元(3)、桔梗皂苷D3(4)、桔梗皂苷F(5)、桔梗皂苷D(6)、去芹糖桔梗皂苷D(7)、桔梗皂苷元B(8)、3-O-β-D-吡喃葡萄糖基远志酸(9)、雷叶木皂苷A(10)、亚油酸(11)、(6Z,9Z)-6,9-十六碳二烯酸(12)、亚油酸甘油酯(13)、(2S)-1-O-(9Z,12Z-十八烷二烯基)-3-O-β-半乳糖基甘油(14)、邻苯二甲酸二丁酯(15)、三烯丙基异氰脲酸酯(16).抗药物性肝损伤活性研究表明,化合物1～3、5～7、9、14能显著抑制对乙酰氨基酚诱导的AML12细胞上清液中丙氨酸氨基转移酶、天门冬氨酸氨基转移酶水平(P＜0.05、0.01).结论 从桔梗总皂苷肠道菌转化产物中成功分离并鉴定了 16个化合物,其中化合物1为新化合物,命名为去芹糖木糖基远志皂苷D;化合物1～3、5～7、9、14表现出显著的抗药物性肝损伤活性,具有抗肝损伤潜力.
Objective To investigate the chemical constituents of the intestinal bacteria transformation products of total saponins from Platycodon grandiflorus and their anti-drug-induced liver injury activity.Methods The compounds were isolated and purified by column chromatography techniques including AB-8 macroporous resin,normal phase silica gel,Sephadex LH-20 gel chromatography,and semi-preparative high-performance liquid chromatography.Their structures were identified based on physicochemical properties,mass spectrometry and nuclear magnetic resonance spectral data.The anti-drug-induced liver injury activities of the isolated compounds were evaluated using an acetaminophen-induced AML 12 cell hepatic injury model.Results A total of 16 compounds were isolated from the intestinal bacteria transformation products of total saponins from P.grandifloras and identified as 3-O-β-D-glucopyranosyloxy-2β,3β,16α,23-tetrahydroxyolean-12-en-28-oic acid-28-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside(1),polygalacin D(2),3-O-β-D-glucopyranosyl platycodigenin(3),platycodin D3(4),platycoside F(5),platycodin D(6),deapio-platycodin D(7),platycodonoid B(8),3-O-β-D-glucopyranosyl polygalacic acid(9),leiyemudanoside A(10),linoleic acid(11),(6Z,9Z)-6,9-hexadecadienoic acid(12),glyceryl linoleate(13),(2S)-1-O-(9Z,12Z-octadecandienyl)-3-O-β-galactosyl glycerol(14),dibutyl phthalate(15),and triallyl isocyanurate(16).Anti-drug-induced liver injury activity study showed that compounds 1-3,5-7,9,and 14 significantly inhibited the levels of alanine aminotransferase and aspartate aminotransferase in acetaminophen-induced AML 12 cell supernatants(P＜0.05,0.01).Conclusion A total of 16 compounds were successfully isolated and identified from the intestinal bacterial transformation products from total saponins of P.grandiflorus.Compound 1 is a new compound named des-apio-xylosyl polygalacin D.Compounds 1-3,5-7,9,and 14 exhibit significant anti-drug-induced liver injury activities,showing potential hepatoprotective effects.
彭昱;钟国跃;曾金祥;梁永红;钟渊涵;万同林;刘光鹏;文丽云;饶建波;梁健;秦倩;张寿文
江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学现代中药制剂教育部重点实验室,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004江西中医药大学中药资源与民族药研究中心,江西南昌 330004
医药卫生
桔梗总皂苷肠道菌转化产物药物性肝损伤去芹糖木糖基远志皂苷D远志皂苷D桔梗皂苷D3
total saponins of Platycodonis Radixintestinal bacterialtransformation productdrug-induced liver injurydes-apio-xylosyl polygalacin Dpolygalacin Dplatycodin D3
《中草药》 2026 (7)
2445-2455,11
国家自然科学基金项目(82160736)江西中医药大学科技创新团队项目(CXTD22002)
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