不同生境烟草及周边植物病毒多样性及田间溯源分析OA

[Objective]The study aimed to identify primary virus species,infection types,and virus host plants of tobacco and surrounding plants in different habitats within Chuxiong tobacco-growing area,Yunnan province.[Method]By RT-PCR technique,nine common tobacco viruses(TMV,CMV,PVY,TVBMV,ChiVMV,PCSV,INSV,TZSV,TSWV)of tobacco and surrounding plants from six different habitats were detected,sequenced,and followed by phylogenetic analysis.[Result]Six types of viruses,namely TMV,CMV,TVBMV,ChiVMV,PCSV and TSWV,were detected in tobacco and surrounding plants from different habitats.Among them,TMV was the predominant virus,with a de-tection rate of 91.7％,followed by TSWV with a detection rate of 85.4％.The compound infection of tobacco and surrounding plants was se-vere,with a compound infection rate as high as 97.9％.The predominant infection combination was TMV+TSWV+ChiVMV.The field virus host plants for the six tobacco virus diseases were as follows,seven types including Dichrocephala auriculata,Artemisia carvifolia,Alter-nanthera philoxeroides,Bidens pilosa,Brassica oleracea,Cucumis sativus and Rosa rugosa for TMV;six types including A.philoxeroides,A.carv-ifolia,Malva verticillata,Capsicum annuum,Phaseolus vulgaris and Oenanthe javanica for ChiVMV;five types including Brassica napus,Citrus reticulata,Equisetum hyemale,B.pilosa and B.oleracea for TSWV;four types including D.auriculata,B.pilosa,A.philoxeroides and B.olera-cea for TVBMV;two types including A.philoxeroides and C.annuum for CMV;two types including E.hyemale and R.rugosa for PCSV.[Con-clusion]The compound infection of virus diseases in tobacco and surrounding plants from different habitats within Chuxiong tobacco-growing area of Yunnan province is severe.In particular,TMV,TSWV and ChiVMV have high detection rates and wide host ranges,which shall be given key attention.During the tobacco-planting period,the removal of virus host plants around tobacco fields and the prevention and control of virus-transmitting insects can be listed as preventive measures for tobacco virus diseases.