长日照条件下二穗短柄草不同发育时期蛋白组学分析OA

[Objective]The dynamic changes in the proteome of Brachypodium distachyon at different developmental stages under long-day conditions were explored and the protein regulatory patterns associated with development and photoperiod-induced flowering were analyzed to provide a theoretical basis for the photoperiod domestication of temperate gramineous crops.[Method]B.distachyon was grown under long-day conditions(LD,16 h light/8 h dark).TMT-based quantitative proteomics technology was used to compare the protein expression profiles in leaves at the seedling stage(DYM),tillering stage(DFN),booting stage(DYS)and heading stage(DCS).[Result]A total of 5705 pro-teins were identified,and 1456 differentially expressed proteins(DEPs)were screened out.With the progression of developmental processes,the number of DEPs increased obviously,and the complexity of the protein-protein interaction network continued to enhance.Among these proteins,NDPK played multiple regulatory roles in plant growth,development,and stress adaptation,while RPL37 and RPL23 may be the key proteins driving the transition from vegetative growth to reproductive growth.GO analysis indicated that DEPs were mainly enriched in func-tional terms such as response to stimulus,response to stress,and antioxidant enzyme activity.KEGG analysis showed that DEPs were primarily enriched in pathways including plant-pathogen interaction,plant MAPK signal transduction,and plant circadian rhythm regulation related to photoperiod.Four proteins of ELF5,CCA1,BAM3 and PHYB encoded by differential genes were screened from photoperiod-related pathways.[Conclusion]ELF5,CCA1,BAM3 and PHYB may be the key genes regulating the normal growth and development of B.distachyon.NDPK participates in the regulation of growth and development by maintaining redox homeostasis,while RPL37 and RPL23 are the key proteins dur-ing its reproductive growth process.