烟草COPT基因家族鉴定及表达特征分析OA

[Objective]The study aimed to comprehensively analyze the characteristics,evolutionary relationship and expression regulation mode of tobacco COPT gene family members,and provide theoretical basis for the subsequent screening of tobacco resistant varieties.[Meth-od]The study systematically identified the COPT gene family members in the tobacco k326 genome and analyzed its physicochemical proper-ties,gene structure,conserved motifs,evolutionary relationships,synteny and duplication analysis,and protein interactions.We also explored the expression patterns of COPT gene family members in different tissues such as roots,young leaves and shoot tips and under apical stress,drought conditions and tobacco blight infestation.[Result]A total of eight COPT genes were identified in the tobacco genome of k326 variety,unevenly distributed on seven chromosomes,all of which were hydrophobic proteins.Eight co-linear gene pairs existed among NtCOPT genes with Ka/Ks values less than 1,and the upstream promoter regions of the genes contained a variety of hormone-,growth-development-,stress-and light-responsive elements.The transcriptomic data indicated that most NtCOPT genes were highly expressed in the young leaf site with some tissue specificity.The expression of genes NtCOPT4,NtCOPT6 and NtCOPT8 was increased under apical and drought treatments;and the expression of most genes was significantly up-regulated after tobacco blight infestation.[Conclusion]Eight NtCOPT gene family members were relatively evolutionarily conserved and had different expression patterns in different tissues,biotic and abiotic stresses,which were hy-pothesized to play important roles in tobacco growth and development,drought resistance,and resistance to tobacco blight mold.The results laid the foundation for further research and utilization of tobacco COPT genes.