Bacillus velezensis-1功能验证及西瓜枯萎病防治转录组分析OA
Functional verification of Bacillus velezensis-1 and transcriptome analysis for Fusarium wilt of watermelon control
[目的]为获得提高西瓜抗枯萎病能力的生防菌株,探究生防菌抑菌物质和接菌后对西瓜抗病反应的影响.[方法]从健康植株根际平板分离筛选优势菌,分类鉴定菌株和生长条件,通过显微观察抑菌效果,结合促生性检测、抑菌物质分泌检测和西瓜接菌后转录组水平检测揭示Bacillus velezensis-1(以下简称BVS-1)抑菌机理.[结果]BVS-1是一株贝莱斯芽孢杆菌(Bacillus velezensis),最适pH为7,最适温度30℃,最适氮源为牛肉浸粉,最适碳源为酵母提取物,最适盐浓度10 g/L.BVS-1平板对峙抑菌率达60％,能有效抑制菌丝生长;扫描电镜观察对峙区域表明BVS-1能引起Fon菌丝破裂、扭曲变形;胞外酶检测发现BVS-1能分泌蛋白酶、淀粉酶和纤维素酶,能合成生长素,具有解钾能力、固氮能力和铁载体运输功能,能挥发产生2,4-二叔丁基苯酚、硬脂酸、十五烷酸等抑菌性有机挥发物,接菌后西瓜的苯丙氨酸代谢、α-亚麻酸代谢、谷胱甘肽代谢途径差异明显,正向调控光合作用-天线蛋白途径.[结论]贝莱斯芽孢杆菌BVS-1对尖胞镰刀菌表现出良好的抑菌效果,显著提升西瓜抗枯萎病能力,具有较好的应用前景,可为西瓜枯萎病的生物防治提供资源.
[Objective]The study aimed to obtain a biocontrol strain that enhanced watermelon resistance to Fusarium wilt of watermelon,and to investigate the antifungal substances produced by the strain and its impact on disease resistance responses in watermelon after inoculation.[Method]Dominant strains were isolated and screened from the rhizosphere of healthy plants.The strain was identified,and its growth condi-tions were determined.Antifungal effects were observed microscopically,and the inhibition mechanisms of Bacillus velezensis-1(hereinafter referred to as BVS-1)were explored through growth-promotion tests,detection of antifungal substance secretion,and transcriptome analysis of watermelon after inoculation with the strain.[Result]BVS-1 was identified as B.velezensis.Its optimal growth conditions were pH 7,30 ℃,beef extract powder as the nitrogen source,yeast extract as the carbon source,and a salt concentration of 10 g/L.The plate confrontation assay showed an inhibition rate of 60％against Fusarium oxysporum f.sp.niveum(Fon),effectively suppressing mycelial growth.Scanning elec-tron microscopy revealed that BVS-1 caused hyphal rupture,distortion and deformation.Extracellular enzyme tests indicated that BVS-1 secre-ted protease,amylase and cellulase.It could synthesize auxin,solubilize potassium,fix nitrogen and produce siderophores.Antimicrobial vola-tile organic compounds such as 2,4-di-tert-butylphenol,stearic acid and pentadecanoic acid were detected.After inoculation,significant differences were observed in watermelon metabolic pathways,including phenylalanine metabolism,α-linolenic acid metabolism,and glutathi-one metabolism,with upregulation of the photosynthesis-antenna protein pathway.[Conclusion]B.velezensis BVS-1 demonstrates strong anti-fungal activity against Fusarium oxysporum,significantly enhances watermelon resistance to Fusarium wilt,and shows promising application potential.This strain can serve as a valuable resource for the biological control of watermelon Fusarium wilt.
钱文浩;郭东升;周鹏飞;陈丽萍
湖州市农业科学研究院,浙江湖州 313000浙江大学农业与生物技术学院,杭州 310000中科院上海植物生理研究所,上海 200030湖州市农业科学研究院,浙江湖州 313000
农业科技
西瓜枯萎病贝莱斯芽孢杆菌胞外酶抑菌性有机挥发物转录组
Fusarium wilt of watermelonBacillus velezensisExtracellular enzymesAntimicrobial volatile organic compoundsTranscriptome
《西南农业学报》 2026 (2)
244-256,13
浙江省蔬菜产业技术团队项目(浙农科发[2023]13号)湖州市科技项目(3305004022141)
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