基于多组学分析水生蔬菜豆瓣菜对镉胁迫逆境的响应OA

[Objective]The study aims to explore the mechanisms underlying watercress(Nasturtium offi-cinale)response to Cadmium(Cd)stress,to provide a reference basis for mitigating Cd toxicity and advancing research on plant resistance to heavy metals.[Method]Hydroponically grown watercress seedlings were treated with 0,0.2,0.8,3.2,and 12.8 mg/L CdCl2 to determine the appropriate Cd concentration.The effects of Cd stress on growth,physiological indices,metabolite levels,and gene expression were investigated.[Result]①Under 0.8 mg/L CdCl2 stress,biomass and photosynthesis of watercress were significantly inhibited.Sulfur(S)content was decreased,while Cd,Fe,Mn,K,and Ca contents were increased.Activities of POD and SOD were declined,whereas MDA levels were increased.Additionally,root vascular bundle area was decreased,cell walls were thickened,and intracellular structures,such as plastids and mitochondria,were damaged.② Cd stress downregulated the expression of the genes associated with the MAPK signaling pathway(133 genes)and phen-ylpropanoid biosynthesis pathway(104 genes),impairing stress responses and cell wall reinforcement.It also suppressed the expression of genes related to glycolysis and gluconeogenesis,affecting energy metabolism,while simultaneously upregulating the expression of genes involved in peroxisome activity,alleviating oxidative dam-age.③ Cd stress inhibited the synthesis of metabolites related to glycolysis,starch,and sucrose metabolism.[Conclusion]0.8 mg/L CdCl2 stress significantly inhibited the growth and photosynthesis of watercress.Tran-scriptomic and metabolomic analyses showed that Cd affected the accumulation of carbon metabolites by inhibit-ing the activity of the antioxidant system and the expression of genes related to the carbon metabolism pathway,ultimately hindering the growth of watercress.