基于ILP标记的50份云南山茶品种遗传多样性分析OA

[Objective]By analyzing the genetic diversity of germplasm from 50 Camellia reticulata variet-ies,this study aims to provide a scientific basis for the innovative use of camellia germplasm resources and the ad-vancement of breeding process.[Method]Molecular marker tests were conducted on 50 Camellia reticulata va-rieties using 25 pairs of screened polymorphic primers,and their genetic diversity,kinship,population structure and principal components were systematically analyzed.[Result]A total of 112 alleles were detected in Camel-lia reticulata varieties,with the number of alleles ranging from 3 to 7 and averaging 4.48.The number of effec-tive alleles averaged 2.44,and the mean values of observed heterozygosity(Ho)and expected heterozygosity(He)were 0.72 and 0.55,respectively.The mean values of Shannon polymorphism index(I)and Nei genetic diversity index(H)were 0.99 and 0.55,respectively,indicating high genetic diversity in Camellia reticulata va-rieties.The average polymorphic information content(PIC)of the primers was 0.52,with 14 pairs of primers having PIC values greater than 0.5,indicating that these primers can effectively reflect genetic diversity.Genetic distances calculated based on NTSYSpc-2.1 showed genetic similarity coefficients of the 50 varieties ranged from 0.61 to 0.92,with'Xiangfei'and'Fengshancha'having the highest genetic similarity,and'Damanao'and'Taohongpao'having the lowest genetic similarity.Based on analysis using Structure software,the 50 Ca-mellia reticulata varieties could be divided into two groups at △K=2,with one group containing 46 varieties.Principal component analysis further revealed that the 50 varieties were highly similar in genetic relationship and basically clustered together.[Conclusion]The 25 selected primer pairs can effectively reflect the genetic diver-sity of Camellia reticulata germplasm(PIC＞0.5).High genetic similarity was observed among the 50 Camel-lia reticulata germplasm,with exceptionally close genetic relationships.