茶树CsMPC基因家族鉴定及胁迫表达分析OA

[Objective]This research aims to investigate the functional roles of the mitochondrial pyruvate carrier(MPC)gene family in tea plant growth,development,and stress responses.[Method]The CsMPC gene family of five tea tree cultivars was identified on a whole-genome scale,and a phylogenetic tree was constructed.ProtParam and ProtScale were used to analyze the physicochemical properties of CsMPC proteins,and MEGA was used to examine their conserved motifs.TMHMM and Robetta were used to predict the transmembrane do-mains and tertiary structures of CsMPC proteins.Three CsMPC genes were cloned from the'Shuchazao'culti-var,and their subcellular localization was analyzed through a transient transformation system combined with PRE-PROTEIN online prediction.Alternative splicing analysis was performed by using TeaAS,and expression patterns of CsMPC genes were explored based on transcriptomic data.The expression responses of CsMPC genes to low temperature,drought,and salt stresses were validated through quantitative PCR(RT-qPCR)with the'Longjing 43'cultivar as the experimental material.[Result]Three CsMPC gene family members were identified in tea trees,belonging to three distinct subgroups:MPC1,MPC2 and MPC3.Sequence similarity and conserved motifs of CsMPC genes varied across tea cultivars,with CsMPC1 showing the highest sequence simi-larity of 31％ to CsMPC2 and 34％ to CsMPC3.The proteins encoded by CsMPC ranged from 93 to 131 amino acids in length,exhibiting hydrophilic/hydrophobic characteristics and transmembrane features in certain culti-vars.Tertiary structure prediction revealed significant structural differences among CsMPC proteins from differ-ent cultivars,suggesting that CsMPC proteins function as transporters in the form of heterodimers.Mitochon-drial localization of CsMPC proteins further supported their role as pyruvate transporters in cellular metabolism.Under stresses including low temperature,drought,and salinity,CsMPC genes responded via alterations in splic-ing events and tissue-specific expression levels.Real-time quantitative PCR results confirmed that the expres-sion patterns of CsMPC genes aligned closely with transcriptomic data.[Conclusion]Three CsMPC genes ca-pable of regulating pyruvate transport and responding to stress were identified in different tea cultivars,offering insights for further exploration of the function and regulatory mechanisms of MPC genes in tea plants.