肉桂醛对小鼠卵泡颗粒细胞雌激素分泌及其凋亡的影响OA
The Effect of Cinnamaldehyde on Estrogen Secretion and Apoptosis of Mouse Ovarian Granulosa Cells
小鼠卵泡颗粒细胞(granulosa cells,GCs)是分泌雌激素的核心细胞.为探究肉桂醛处理对小鼠卵泡GCs雌激素分泌及凋亡的影响.本研究选用3周龄SPF级KM雌鼠,分离获取原代小鼠卵泡GCs并将其分为空白对照组及5×10-5 mol·L-1、10×10-5 mol·L-1肉桂醛处理组,每组3个重复.首先通过转录组测序分析肉桂醛处理后小鼠卵泡GCs的基因表达情况和差异表达基因富集情况;再通过ELISA、CCK-8、流式细胞术和RT-qPCR明确肉桂醛对小鼠GCs雌激素分泌及其凋亡的影响.测序结果表明,5×10-5 mol·L-1肉桂醛处理的小鼠卵泡GCs的差异表达基因富集于PI3K-Akt信号通路、肿瘤相关通路、糖尿病并发症中的AGE-RAGE信号通路和孕酮介导的卵母细胞成熟等.试验结果表明,5×10-5 mol·L-1肉桂醛处理小鼠卵泡GCs能通过显著上调StAR、CYP19A1和CYP11A1的mRNA相对表达水平促进雌激素分泌;与对照组相比,5×10-5和10×10-5 mol·L-1肉桂醛处理均导致小鼠卵泡GCs不同程度的凋亡,5×10-5 mol·L-1肉桂醛处理显著上调CHOP、IRE1、ATF6和PERK的mRNA相对表达水平,10×10-5 mol·L-1肉桂醛处理显著上调了 CHOP和ATF6的mRNA相对表达水平.综上所述,5× 10-5 mol·L-1肉桂醛可能是通过PI3K-Akt信号通路上调类固醇激素合成酶的表达从而促进小鼠卵泡GCs的雌激素分泌;5×10-5和10×10-5 mol·L-1肉桂醛处理浓度均会通过ERS途径导致GCs凋亡.
Ovarian granulosa cells(GCs)of mice are the core cells responsible for estrogen secretion.This study aimed to investigate the effect of cinnamaldehyde treatment on estrogen secretion and apoptosis of mouse ovarian GCs.In this study,3-week-old SPF-grade female KM mice were selected.The mouse ovarian GCs were isolated and divided into a blank control group,a 5× 10-5 mol·L-1 cinnamaldehyde treatment group,and a 10×10-5 mol·L-1 cinnamaldehyde treatment group.Each group had 3 replicates.Firstly,this study analyzed the gene expression profiles of cinnamaldehyde-treated mouse follicular GCs and the enrichment of differentially expressed genes(DEGs)using transcriptome sequencing analyses.Then,ELISA,cell counting Kit-8(CCK-8),flow cytometry,and RT-qPCR were employed to clarify the effect of cinnamaldehyde on estrogen secretion and apoptosis of mouse GCs.The sequencing results showed that the DEGs in mouse ovarian GCs treated with 5×10-5 mol·L-1 cinnamaldehyde were enriched in the PI3K-Akt signaling pathway,tumor-related pathways,AGE-RAGE signaling pathway in diabetic complications,and progesterone-mediated oocyte maturation,among others.The experimental results showed that treatment of mouse ovarian granulosa cells GCs with 5×10-5 mol·L-1 cinnamaldehyde could promote estrogen secretion by significantly upregulating the relative mRNA expression levels of StAR,CYP19A1,and CYP11A1.Compared with the control group,treatment with 5×10-5 mol·L-1 and 10×10-5 mol·L-1 cinnamaldehyde both induced apoptosis of mouse ovarian GCs to different degrees.The 5×10-5 mol·L-1 cinnamaldehyde treatment significantly upregulated the relative mRNA expression levels of CHOP,IRE1,ATF6,and PERK,while 10×10-5 mol·L-1 cinnamaldehyde treatment significantly upregulated the relative mRNA expression levels of CHOP and ATF6.In conclusion,5×10-5 mol·L-1 cinnamaldehyde may promote estrogen secretion in mouse ovarian GCs by upregulating the expression of steroid hormone synthases via the PI3K-Akt signaling pathway.Additionally,both 5×10-5 mol·L-1 and 10×10-5 mol·L-1 cinnamaldehyde can induce GCs apoptosis through the endoplasmic reticulum stress(ERS)pathway.
郭雨欣;林双秀;傅昌盛;杨洋;王登峰;郭盼盼;吴海燕;殷光文;王磊
福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002福建农林大学动物科学学院,福州 350002
农业科技
肉桂醛小鼠卵泡颗粒细胞雌激素类固醇激素合成酶内质网应激
cinnamaldehydemouse ovarian granulosa cellsestrogensteroid hormone synthasesendoplasmic reticulum stress
《畜牧兽医学报》 2026 (4)
2048-2059,12
国家自然科学基金(32102734)福建省中青年教师教育科研项目(JAT210659)福建农林大学校科技创新专项基金项目(KFB25008A)
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