狮头鹅基因组杂合区域的鉴别及其与出栏体重、体尺性状的关联分析OA

This study aimed to analyze the genome-wide characteristics of runs of heterozygosity(ROHet)in the Lion-head goose population and explore their association with body weight and body size traits at slaughter age,which provides references for the conservation of genetic diversity and molecular breeding of local goose breeds.Body weight and 11 body size traits of 504 120-day-old Lion-head geese were collected,and next-generation whole-genome sequencing(10×)was carried out.The software such as BWA v0.7.17 and GATK v4.2.3 were used to align the sequencing data and detect SNPs which obtained 11 005 323 high-quality SNPs.The R package detectRUNS was used to identify ROHet and ROHet islands.The GEMMA software was used to conduct a genome-wide association study(GWAS)of ROHet with body weight and 11 body size traits,and then gene annotation and GO enrichment analysis were performed on significant ROHets.A total of 27 329 ROHets were identified,among which short-fragment ROHets(1-2 Mb)accounted for 82.72％.We detected 9 ROHet islands which were involved in 301 genes.The GO analysis of these 301 genes revealed 3 signaling pathways involved in growth and reproduction and 13 candidate genes including DAG1,INHBA,SOS1,et al.The GWAS of body weight and body size traits and ROHets identified 54 chromosome-wide significant ROHets which were involved in 867 genes.Then the GO analysis of these 867 genes identified 4 signaling pathways involved in growth and reproduction and 10 candidate genes including LRP6,GLI3,ZIC1,et al.This study systematically analyzed the characteristics of ROHet in Lion-head geese.And identified 7 biological pathways and 23 candidate genes associated with body weight and body size traits of Lion-head geese.It provides theoretical support for the research on the molecular mechanism and genetic improvement of body weight and body size traits in Lion-head geese.