高抗逆性纤维素酶产生菌降解复方药渣研究OA

Objective Screening strains with high stress resistance cellulase-production for improve the resource utilization rate of com-pound medicinal residues.Methods High-yield cellulase strains were screened from the rotten compound medicine residues,and were identified through ITS sequence analysis.The fermentation process was optimized through single factors and response surfaces.The per-formance of the strain in degrading compound medicine residues was investigated.Results A fungal strain CKT2 with high cellulose-producing and tolerance to the residue of compound medicine residues was screened,and ITS sequence analysis identified as Penicillium sp.The stress resistance results showed that CKT2 could grow in pH 5.0,250 g/L PEG6000,and 60 μg/mL extracts.Through single-factor and response surface optimization,the optimal cellulase production parameters were:the addition residues 2.8%,inoculation amount 1.43%,initial pH 4.60,rotational speed 150 r/min.Under this optimal condition,the filter paper enzyme,endoglucanase and xylanase activities of Penicillium sp.CKT2 were 13.18 U/mL,107.91 U/mL and 217.91 U/mL,respectively.The degradation rate of Penicillium sp.CKT2 enzymatic hydrolysis compound medicine residues was 45.87%.Conclusion Penicillium sp.CKT2 has good envi-ronmental adaptability and high yield of cellulase,and has a good degradation effect on compound medicine residues.