慢性淋巴细胞白血病骨髓细胞遗传学检测中添加脂多糖提高核型分析效能的应用研究OA

Objective To investigate the application value of lipopolysaccharide(LPS)in cytogenetic detection of chronic lympho-cytic leukemia(CLL),and improve the efficiency of chromosome karyotype analysis in CLL.Methods A total of 93 bone marrow samples diagnosed with CLL and submitted to KingMed Diagnostics Shanghai Laboratory from June 2020 to December 2023 were enrolled.Each sample was inoculated into two culture media simultaneously:the control medium without LPS,while the experi-mental medium supplemented with LPS(83 µg/ml).Chromosome karyotype analysis was performed using G-banding technique.The culture success rate,detection rate of abnormal karyotypes,and detection rate of complex karyotypes were compared between the experimental group and the control group.Results The success rates of chromosome culture in the experimental group and the control group were 91.40%and 87.10%,respectively,and the detection rates of complex karyotypes were 18.82%and 9.88%,respectively,without statistically significant differences(χ2=0.90,2.68,all P＞0.05).The detection rates of abnormal karyotypes were 56.47%and 28.40%,respectively,with a statistically significant difference(χ2=13.36,P=0.000 3).Five samples with abnormal karyotypes were simultaneously detected by fluorescence in situ hybridization(FISH),and the results showed that the abnormal karyotypes identified by LPS stimulated chromosome analysis were consistent with FISH results.Conclusions Application of LPS can improve the detection rate of abnormal karyotypes for CLL,and help identify more complex karyotypes,providing more useful information for clinical diagnosis,treatment and prognosis stratification of CLL.LPS can be widely used in karyotype analysis for CLL.