dRVVT标准化比值和Rosner指数在多点稀释法测定狼疮抗凝物阳性血浆内源性凝血因子活性中的应用研究OA

Objective To explore the role of the dilute Russell viper venom time(dRVVT)normalized ratio and the Rosner index in determining the optimal dilution ratio for detecting endogenous coagulation factor activity in lupus anticoagulant(LA)-positive plasma through multi dilution analysis(MDA).Methods 70 specimens from patients at the First Affiliated Hospital of Xi'an Jiaotong Univer-sity from May 2024 to August 2024 that tested positive for lupus anticoagulant(LA)via dRVVT were selected and divided into three groups based on the dRVVT normalized ratio:the low-value group(1.2～1.5),the medium-value group(1.5～2.0),and the high-value group(≥2.0).The activity of endogenous coagulation factors was measured using MDA,and the results of factor activity at different dilutions were analyzed.Concurrently,the immediate correction test for activated partial thromboplastin time(APTT)was performed on 25 of these cases to analyze the correlation between the Rosner index and the optimal dilution ratio.Results For the low-value group,both the undiluted sample and a 1:22 dilution could be selected.For the median value group,a dilution of 1:22 or 1:23 could be selected.For the high-value group,a 1:23 dilution was required as the initial dilution.No significant correlation was found between the Rosner index and the dRVVT normalized ratio or the optimal dilution ratio(r=-0.095,P=0.651;r=0.197,P=0.346).Conclusions The dRVVT normalized ratio can guide the selection of the optimal dilution ratio for the detection of endogenous coagulation factors in LA-positive plasma,and there is no significant correlation between the Rosner index and the optimal dilution ratio.