丁公藤超高效液相色谱指纹图谱建立及含量测定OA

[Objective]To establish an ultra-high performance liquid chromatography(UPLC)fingerprint and a multi-component content determination method for Erycibe schmidtii Craib,and to evaluate the quality of 30 batches of this medicinal material.[Methods]Using photodiode array UPLC(UPLC-PDA),separation was performed on an Agilent Eclipse XDB-C18 column(2.1 mm×100 mm,1.8 μm)with gradient elution using 0.1%formic acid in water(A)and acetonitrile(B)as the mobile phase.The UPLC fingerprint of E.schmidtii was established,common peaks were identified,and their contents were determined.[Results]The UPLC fingerprints of 30 batches of E.schmidtii were established,and 12 common peaks were obtained.Nine chemical components were identified,showing good linear relationships with peak areas within certain concentration ranges(r≥0.999).The instrument precision,method repeatability,and stability of the test solution were satisfactory,with RSD values all below 5.00%.The average sample recovery rates ranged from 95.3%to 103.0%.[Conclusion]The established UPLC fingerprint and multi-component content determination method for Erycibe schmidtii Craib is stable and reliable,and can be used for subsequent quality control and research of this medicinal material.